Generator

Part:BBa_K103020:Experience

Designed by: Michael Lower   Group: iGEM08_Warsaw   (2008-10-09)
Revision as of 20:01, 27 October 2008 by Keton (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K103020

'Hunter and prey' selection system

This part was used as core of prey generator device. It was fused with protein ZSPA-1 BBa_K103004. Resulting fusion was named His_omega_Z

The fusion was overexpressed in Roseta strain. Due to ability of protein Z to form aggregates His_omega_Z was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega purified] simply by collecting post-sonication debris. The fusion was added to culture media and used in selection experiments. Details here and here.

User Reviews

UNIQ7ff4eccb17704434-partinfo-00000000-QINU UNIQ7ff4eccb17704434-partinfo-00000001-QINU