Signalling
Subtilin P

Part:BBa_K104001:Design

Designed by: The Newcastle 2008 iGEM team   Group: iGEM08_Newcastle_University   (2008-10-24)
Revision as of 10:05, 25 October 2008 by Mtaschuk (Talk | contribs) (→‎References)

Sensor for small peptide Subtilin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A terminator was included after the spaRK to prevent read through. The native SpaRK promoter is controlled by the sigma factor sigma-H and induced at the end of exponential phase. The regulation by sigma-H was left unmodified.


Source

The two component system, its promoter and RBS's, and the pSpaS promoter sequence are all derived from the genome of Bacillus subtilis ATCC 6633.

References

C Klein, C Kaletta, N Schnell and K D Entian (1992). Analysis of genes involved in biosynthesis of the lantibiotic subtilin. Appl Environ Microbiol. 58(1): 132-142.


C Klein, C Kaletta and K D Entian (1993). Biosynthesis of the lantibiotic subtilin is regulated by a histidine kinase/response regulator system. Appl Environ Microbiol. 59(1): 296-303.