Signalling
Subtilin P

Part:BBa_K104001:Design

Designed by: The Newcastle 2008 iGEM team   Group: iGEM08_Newcastle_University   (2008-10-24)

Sensor for small peptide Subtilin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A terminator was included after the spaRK to prevent read through. The native SpaRK promoter is controlled by the sigma factor sigma-H and induced at the end of exponential phase. The regulation by sigma-H was left unmodified.

This Part was built from virtual parts, computationally modeled and then synthesised in its entirety by GenScript. Thus, there are no scars where virtual parts have been joined.

Source

The two component system, its promoter and RBS's, and the pSpaS promoter sequence are all derived from the genome of Bacillus subtilis ATCC 6633.

References

C Klein, C Kaletta, N Schnell and K D Entian (1992). Analysis of genes involved in biosynthesis of the lantibiotic subtilin. Appl Environ Microbiol. 58(1): 132-142.


C Klein, C Kaletta and K D Entian (1993). Biosynthesis of the lantibiotic subtilin is regulated by a histidine kinase/response regulator system. Appl Environ Microbiol. 59(1): 296-303.