Device
TARS-LacI
Part:BBa_K108030:Design
Designed by: Shan Lin Group: iGEM08_Tsinghua (2008-10-23)
TARS-LacI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 31
Design Notes
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Source
LacI Operator sequence was designed according to reference 1.
TARS sequence was designed according to reference 2.
LacI and T7 terminator was cloned from pET-15b(Novagen).
LVA sequence was designed according to reference 3.
References
1. Bacterial Transcriptional Regulators for Degradation Pathways of Aromatic Compounds. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS. 2004, 68(3):474–500.
2. An Artifical Aptazyme-Based Riboswitch and its Cascading System in E.coli. ChemBioChem. 2008, 9:206-209.
3. New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 1998, 64(6):2240–2246.