Part:BBa_K3215013

Tse2 Repressed by Arabinose Kill Switch

This kill switch was created to enable its user to reduce an organism's growth rate in a medium without arabinose. It is consisted of parts BBa_K259007, BBa_K327018, BBa_B0015, BBa_R0051, BBa_J61100 and BBa_K314200.

Usage and Biology

Considering a system where the survivel of an organism is inadequate, this BioBrick shows a way of handling the issue. When this mechanism is present, the presence of L-(+)-Arabinose will enhance the transcription of cI, which will repress the expression of the Tse2 toxin, enabling the survival of the organism. On the other hand, when the former is absent, the transctiption of cI will be inhibited, which in turn will activate the expression of Tse2 toxin, repressing the survival rate of those organisms.

Fig. 1. BBa_K3215013 scheme.

Characterization

To characterize and validate our part, two different approaches were made:

Wet lab experiment validation

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 294
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1532
  Illegal NgoMIV site found at 1589
  Illegal NgoMIV site found at 1714
  Illegal AgeI site found at 125
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 107