Regulatory
P-erm

Part:BBa_K3183000

Designed by: Natasha Cooke   Group: iGEM19_Oxford   (2019-09-20)
Revision as of 10:51, 20 October 2019 by Dschramm (Talk | contribs) (→‎Characterisation)


Erythromycin Constitutive Promoter

P-erm is a constitutive promoter which can be used in Lactobacillus reuteri 10023C, and may have uses in other Lactobacillus species. It has also been shown to be functional in E. coli.

The promoter is derived from the erythromycin ribosomal methylase (ermB) promoter from the broad-host range plasmid pAMβ1 isolated from Enterococcus faecalis.1 It was subsequently characterised in six strains of Lactobacillus reuteri and Lactococcus lactisspp. cremoris MG1363.2


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterisation

This part was characterised in the composite part BBa_K3183100. Data for this part is shown below.

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Categories
Parameters
None