Signalling

Part:BBa_K137018

Designed by: Doug Tischer   Group: iGEM08_Caltech   (2008-07-15)
Revision as of 17:21, 17 October 2008 by Jkm (Talk | contribs)

AHL receiver + GFP

This parts produces GFP in response to detecting an acylhomoserinelactone (AHL). It was made to check that the RBS behind the luxR reciever was functional.


Usage and Biology

When measured using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/Flow_cytometry flow cytometry protocol], K137018 in pSB1A2 in DH10B produces the response curve shown below. 100nM AHL caused very slow growth, leading to poor protein expression.

K137018.gif

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005
    Illegal BsaI.rc site found at 1732


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Categories
Parameters
None