Part:BBa_K137018

AHL receiver + GFP

This parts produces GFP in response to detecting an acylhomoserinelactone (AHL). It was made to check that the RBS behind the luxR reciever was functional.

Usage and Biology

When measured using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/Flow_cytometry flow cytometry protocol], K137018 in pSB1A2 in DH10B produces the response curve shown below. 100nM AHL caused very slow growth, leading to poor protein expression.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1005
  Illegal BsaI.rc site found at 1732
  

Functional Parameters: Austin_UTexas

Burden Imposed by this Part:

Burden Value: 16.8 ± 8.2%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This part exhibited a significant burden. Users should be aware that BioBricks with a burden of >20-30% may be susceptible to mutating to become less functional or nonfunctional as an evolutionary consequence of this fitness cost. This risk increases as they used for more bacterial cell divisions or in larger cultures. Users should be especially careful when combining multiple burdensome parts, as plasmids with a total burden of >40% are expected to mutate so quickly that they become unclonable. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.