Part:BBa_K2922038

The colicin-N operon under T7 promoter control with a gfasPurple chromoprotein reporter

Summary

This is a composite part consist of a T7 promoter (BBa_K525998), CDS of colicin-N (BBa_K2922027), CDS of colicin-N immunity protein (BBa_K2922028), CDS of lysis protein (BBa_K2922029) and a gfasPurple chromoprotein reporter(BBa_K1033917) . Each CDS has an RBS (BBa_B0034) behind. T7 promoter could be induced by IPTG or lactose in Escherichia coli BL21 strain and then express all proteins mentioned. E coli that can`t express colicin-N immunity protein would be killed by colicin-N, colicin-N immunity protein is used to protect itself from attack of extracellular colicin-N and lysis protein helps the release of colicin-N. The gfasPurple chromoprotein reporter is used to present the growth curve specifically of strain which contains this part. This part is constructed in the aim of achieve our “aggressive” design.

Ientification

Fig.1 The growth curve of E coli BL21 strain which contained BBa_K2922038 carried by pSB1C3. OD600, OD577 and OD458 is detected to specifically show its growth status

Fig.2 The co-cultural growth curve of E coli BL21 strains which contained BBa_K2922038 or BBa_K1033914. OD600, OD577 and OD458 is detected to specifically track each strans` growth status.IPTG is added in 2h to induced the expression of colicin.

Fig.3 The co-cultural growth curve of E coli BL21 strains which contained BBa_K2922038 or BBa_K1033914. OD600, OD577 and OD458 is detected to specifically track each strans` growth status.This is control group that IPTG is not added.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1938
  Illegal NheI site found at 1961
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 598
  Illegal AgeI site found at 1602
  
• 1000
  COMPATIBLE WITH RFC[1000]