Reporter

Part:BBa_K2943901

Designed by: Dror Hadas   Group: iGEM19_TAU_Israel   (2019-10-14)
Revision as of 15:01, 19 October 2019 by May 1 (Talk | contribs)


Improved mRFP

This sequence is part of a composite part (https://parts.igem.org/Part:BBa_K2943902) that aims to improve an existing composite part (https://parts.igem.org/Part:BBa_K608014).
This sequence is based on the monomer RFP (mRFP, https://parts.igem.org/Part:BBa_E1010) with several mutations inside to optimize expression in our composite part. Mutations were achieved by the program VienneRNA and optimize the mRNA folding energy to gain more efficient translation and expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None