RNA
Part:BBa_K2923002:Design
Designed by: claire husser Group: iGEM19_Strasbourg (2019-09-12)
aptazyme theophylline inactif
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
=Aptazyme is composed of three different part : =Theo5 as a communication module, =P1 as a twister ribozyme mutated in the 14 an 15 position to inhibit the RNA cleavage activity of the ribozyme part =Aptamer module specific to theophylline.
Source
Felletti, M., Stifel, J., Wurmthaler, L.A., Geiger, S., and Hartig, J.S. (2016). Twister ribozymes as highly versatile expression platforms for artificial riboswitches. Nature Communications 7