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Regulatory
miR663b-ta
Part:BBa_K3179000:Design
Designed by: Jiaxian Xu Group: iGEM19_SYSU-CHINA (2019-10-13)
miR663b-target
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We constructed miR663b-target with 2kt-E1A and miR885-5p-target so that E1A cannot be translated if a high level of miRNA663b existing in the cell.
Source
MiRNA 663b-target is constructed bearing four repeats of miRNA 663b, and each repeat added with ATG. Extra bases which can be devisable by three were added in order to separate ATGs.