Composite

Part:BBa_K137079:Experience

Designed by: Doug Tischer   Group: iGEM08_Caltech   (2008-08-19)
Revision as of 19:40, 4 September 2008 by Jkm (Talk | contribs) (→‎Applications of BBa_K137079)

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Please enter how you used this part and how it worked out.

Applications of BBa_K137079

Applications of BBa_K137068

  • KatG+LAA is functional
    • Strain: SN0037 (DE katE, katG)
    • Media: M9 + 2% glucose
    • KatG effectively protects E. coli from exogenous hydrogen peroxide. When expressed behind F2622 and induced with 100nM acylhomoserine lactone, the minimum inhibitory concentration (MIC) of SN0037 for hydrogen peroxide increases from 0.3 mM to 3mM. The MIC is defined as the concentration of hydrogen peroxide needed to prevent overnight turbid growth of a 1:50 dilution of a stationary phase culture.
Hydrogen peroxide (mM) +AHL (100mM) -AHL
0.0 Y
0.3 Y
1.0 Y
3.0 Y
10.0 N

User Reviews

UNIQ8c7aa38f84b8eded-partinfo-00000000-QINU UNIQ8c7aa38f84b8eded-partinfo-00000001-QINU