Composite

Part:BBa_K137079:Experience

Designed by: Doug Tischer   Group: iGEM08_Caltech   (2008-08-19)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K137079

KatG+LAA is functional

  • Strain: SN0037 (DE katE, katG)
  • Media: M9 + 2% glucose
  • KatG effectively protects E. coli from exogenous hydrogen peroxide. When expressed behind F2622 and induced with 100nM acylhomoserine lactone, the minimum inhibitory concentration (MIC) of SN0037 for hydrogen peroxide increases from 0.3 mM to 3mM. The MIC is defined as the concentration of hydrogen peroxide needed to prevent overnight turbid growth of a 1:50 dilution of a stationary phase culture.


Hydrogen peroxide (mM) +AHL (100nM) -AHL
0.0 Y Y
0.3 Y Y
1.0 Y N
3.0 Y N
10.0 N N

Y = Turbid growth after overnight incubation. N = No turbid growth after overnight incubation.

User Reviews

UNIQ7843bb8e8ef7202b-partinfo-00000000-QINU UNIQ7843bb8e8ef7202b-partinfo-00000001-QINU