Coding

Part:BBa_K2684006

Designed by: Yihao Zhang   Group: iGEM18_SHSBNU_China   (2018-10-08)
Revision as of 21:27, 17 October 2018 by Eehow (Talk | contribs)

CsgA-SpyTag

CsgA fused with SpyTag by 2xGGGGS linker This part was improved from <a href="https://parts.igem.org/Part:BBa_K1583000">Part BBa_K1583000</a>.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Overview

We added a SpyTag sequence which fused after csgA gene, creating csgA-spytag (BBa_K2684006). With SpyTag, CotA laccase can be fixed onto the biofilm by forming a covalent bond SpyTag-SpyCatcher.

Gene csgA found in the genome of MG1655 wild type is capable of forming biofilm. Using CRISPR, we knocked out gene csgA on MG1655’s genome creating ΔMG1655 strain. The cell ΔMG1655 would then be used as chassis cell. Gene csgA was fused into plasmid pET28a.

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