Reporter

Part:BBa_K2610042

Designed by: Charlotte de Ceuninck van Capelle, Paula Regal   Group: iGEM18_Leiden   (2018-10-10)
Revision as of 01:57, 18 October 2018 by JazzyDeWaard (Talk | contribs) (→‎Usage and Biology)


pGapA-spisPink

Promoter GapA is naturally occurring constitutive promoter in Escherichia coli.

Usage and Biology

We, iGEM Leiden 2018, have combined this naturally occurring constitutive promoter ([BBa_K1033932) from Escherichia coli with the pink chromoprotein spisPink ([BBa_K592009) in order for us to compare it to our novel stress promoter+chromoprotein constructs.

Contrary to our stress promoters, this promoter has a constitutive high basal expression level. In contrast to many synthetic promoters, this novel promoter provides an indication of high levels of natural expression. Therefore, this promoter can be used as a future positive control of reporter production - such as GFP - in a natural situation. Moreover, GapA being a natural occurring promoter means that there are still possibilities for optimization through radiation. This way variations of this promoter can provide access to an even wider spectrum of colors.


T--Leiden--chromo.png

Figure 1. Constitutively expressed pGapA-AmilCP compared to non-transformed DH5a and pGapA-spis-Pink (pink chromoprotein)

References

Munjal, N., Jawed, K., Wajid, S., & Yazdani, S. S. (2015). A constitutive expression system for cellulase secretion in Escherichia coli and its use in bioethanol production. PloS one, 10(3), e0119917.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 140


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Categories
Parameters
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