Cell
YYC912
Part:BBa_J45999
Designed by: MIT iGEM 2006 Group: iGEM06_MIT (2006-10-30)
E. coli strain YYC912; Odor-free chassis
Escherichia coli strain [http://cgsc2.biology.yale.edu/Strain.php?ID=64826 YYC912]. Biosafety level 1 organism derived from Escherichia coli K12.
BBa_J45999 has a mutation in the tnaA gene which encodes a tryptophanase which converts tryptophan to indole. Indole is responsible for the foul stench of E. coli so tnaA mutants can have reduced indole levels and thus reduced foul odor.
Usage and Biology
- CGSC#: 7602
- Strain Designation
- Designation: YYC912
- Source Person: Cronan & Chang
- Choice: 1
- Sex(Hfr,F+,F-,or F'): F-
- Mutations
- DE(argF-lac)169
- LAM-
- poxB15::lacZ::CmR (Note chloramphenicol resistance)
- IN(rrnD-rrnE)1
- rph-1
- tnaA5
- No. of Muts Carried: 6
- Special Uses: lac fusion-stat.phase promoter
- Has_Partner_Isogenic_WRT: poxB15::lacZ::CmR
- Isogenic_Partner_Is: ZK126
- References Chang-1994
- ID#: 64826
<biblio>
- Chang-1994 pmid=8022274
</biblio>
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//function/odor
s
//function/odor
s
None |