1 Registry Star
Generator
ATF1 gener
Part:BBa_J45199:Experience
Designed by: André Green II Group: iGEM06_MIT (2006-10-28)
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how you used this part and how it worked out.
Applications of BBa_J45199
Banana odor based reporter of transcriptional signal.
User Reviews
UNIQ779f9dec7e13b27f-partinfo-00000000-QINU
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BBa_J45199 was successfully used to produce banana odor under the control of BBa_R0040 and BBa_J45992in the banana odor generators BBa_J45200 and BBa_J45250, respectively. |
UNIQ779f9dec7e13b27f-partinfo-00000007-QINU
Characterization
Transcriptional control of banana odor enzyme generator
The ATF1 enzyme generator is used in both the BBa_J45200 and BBa_J45250 banana odor biosynthetic devices.
Growth dependent banana odor production
To demonstrate growth phase dependent banana odor production, we compared the behavior of constitutive and stationary phase dependent banana odor generators (A and B, respectively). We measured isoamyl acetate concentrations of cultures of the constitutive and stationary phase banana odor generators at different culture cell densities (OD600nm) (C). As expected, the stationary phase banana odor generator produced very little isoamyl acetate at low cell densities but its isoamyl acetate production increased with cell density. By comparison, the constitutive banana odor generator produced more isoamyl acetate at lower cell densities than the stationary phase banana odor generator. To visually aid comparison of the two odor generators, an empirical fit to the data for each device is shown.
To demonstrate growth phase dependent banana odor production, we compared the behavior of constitutive and stationary phase dependent banana odor generators (A and B, respectively). We measured isoamyl acetate concentrations of cultures of the constitutive and stationary phase banana odor generators at different culture cell densities (OD600nm) (C). As expected, the stationary phase banana odor generator produced very little isoamyl acetate at low cell densities but its isoamyl acetate production increased with cell density. By comparison, the constitutive banana odor generator produced more isoamyl acetate at lower cell densities than the stationary phase banana odor generator. To visually aid comparison of the two odor generators, an empirical fit to the data for each device is shown.