Coding
SAMT
Part:BBa_J45001:Design
Designed by: Kate Broadbent Group: iGEM06_MIT (2006-06-07)
SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 577
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 901
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 421
Illegal NgoMIV site found at 425 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 471
Illegal SapI.rc site found at 75
Design Notes
- No BioBrick restriction enzyme cut sites in coding region
- Changed natural stop codon to TAA TAA
- Constructed by PCR
Forward primer
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GAC AAA ACA AAC ACA AAA G -3'
Reverse primer
5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAc tct cgc ctt gtc atg gaa ata g -3'
Internal sequencing primer
5'- gga atg ctg cta tat ttg gg -3'
Source
SAMT gene from Antirrhinum majus (snapdragon flower) ([http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=23957317 GenBank AF515284])
References
<biblio>
- Negre-ArchBiochemBiophys-2002 pmid=12361714
</biblio>
- [http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6 MIT iGEM 2006 notebook entry]