Composite
Part:BBa_K2258003
Designed by: Jyothish S Group: iGEM17_IISER-Pune-India (2017-10-27)
pCAT_AmilCP
It expresses AmilCP constitutively under pCAT promoter PCAT promoter (BBa_I14033) Composite Amil CP (BBa_K1357009)
Usage and Biology
This part is useful as constitutively expressed a reporter.
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iGEM17_IISER-Pune-India: Expression of chromoproteins. The images above show E coli constitutively expressing amilCP BBa_K592009 (blue), tsPurple BBa_K1033906 (purple) and RFP BBa_E1010 (red).
Photometric Characterization
iGEM17_IISER-Pune-India: Absorbance of E.coli culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of E.coli constitutively expressing AmilCP BBa_K592009 (blue) with an absorption peak at 590nm.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//awards/composite_part
//cds/reporter/chromoprotein
//chassis/prokaryote
//cds/reporter/chromoprotein
//chassis/prokaryote
biology | constitutive reporter |
chassis | E.coli |
color | Blue |
emission | 590nm |