Part:BBa_I732954

Ptemplate1 + RBS.ARL4A0604

Genotype	Character	Name
Top10/pT-TERM	So not express any repressors	CR00
Top10/pT-ARL4A0604	Constitutively express ARL4A0604	CR01
Top10/pT-ARL2A0203	Constitutively express ARL2A0203	CR10
Top10/pTet-ARL4A0604-ARL2A203	Constitutively express ARL4A0604 and ARL2A0203	CR11

• RS 01:25, 14 September 2008 (UTC): Note that this plasmid has been split into a plasmid backbone and part. The plasmid backbone is BBa_I732952 and the part is I732815. The physical DNA associated with this part has sequence BBa_I732952-I732815. This part needs to be deleted and the physical DNA remapped to the correct part number.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal suffix found in sequence at 1105
  Illegal EcoRI site found at 3240
  Illegal XbaI site found at 3405
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3240
  Illegal SpeI site found at 1106
  Illegal PstI site found at 1120
  Illegal NotI site found at 1113
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3240
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal suffix found in sequence at 1106
  Illegal EcoRI site found at 3240
  Illegal XbaI site found at 3405
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3240
  Illegal XbaI site found at 3405
  Illegal SpeI site found at 1106
  Illegal PstI site found at 1120
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2279
  Illegal BsaI.rc site found at 3264