![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K313002
Designed by: Kohaku So, Takashi Hiroi, Megumi Iseki, Mami Nakatake, Ryosuke Kamei, Ryo Kariyazono Group: iGEM10_UT-Tokyo (2010-10-14)
flpe DNA recombinase
Flpe is a site-specific DNA recombinase that recognizes FRT sequence. Flpe was derived from yeast site-specific DNA recombinase Flp. (Buchholz, et al.,Improved properties of FLP recombinase evolved by cycling mutagenesis. Nature. Biotechnology, 16:657-662 ( 1998 ))
We successfully removed the internal EcoRI and SpeI sites using site-directed mutagenesis.
Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.
Improvements
• FLPe + NLS: BBa_K2294000
Sequence and Features
[edit]
Categories
Parameters
//function/recombination/flp
None |