DNA

Part:BBa_K2440014

Designed by: Qijie Xu   Group: iGEM17_NUDT_CHINA   (2017-10-22)
Revision as of 01:00, 31 October 2017 by MINXIAO (Talk | contribs) (→‎Usage and Biology)


miR-195 target sequence

It is the target sequence of miR-195, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-195 in an Ago2 dependent manner, that is, knockdown of miR-195 was achieved by transfecting cells with miRNA locker.

Hsa-miR-195 was firstly identified in lung, skin and kidney of mouse by cloning from mouse tissues.1

MicroRNA-195 has been reported as a cancer-related microRNA in many human cancers. Researchers find that miR-195 directly targets the fatty acid synthase enzyme and negatively regulates the expression of fatty acid synthase and finally demonstrated that MiR-195 inhibits the proliferation and invasion of pancreatic cancer cells by targeting the fatty acid synthase2.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.

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Categories
Parameters
None