Coding
Part:BBa_K2316001:Design
Designed by: Shaw Kar Ming Group: iGEM17_NTU_SINGAPORE (2017-10-23)
∆RuvCIII-2 ∆HNH ∆REC2 Sp-dCas9 Enhanced
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 251
Illegal BglII site found at 804
Illegal BamHI site found at 1622
Illegal XhoI site found at 2578 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1966
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2112
Illegal BsaI site found at 2774
Illegal BsaI.rc site found at 1027
Design Notes
Created with Gibson
Source
This part is an improvement of BBa_K2316000