Part:BBa_K2446039
ZF_21-16KRAB
This part is one of our mammalian synthetic transcription factors (SynTFs) based on Cys2-His2 zinc finger 21-16 (ZF_21-16) as protein chassis. [1]. ZF_21-16_KRAB (or TF-KRAB-3 in our project) containing three core domains from N-terminal to C-terminal: ZF_21-16 DNA binding domain, nuclear location sequence and KRAB transcription regulating domain [2].And a (G4S) linker was added between DBD and NLS for providing region flexibility [3]. ZF_21-16-DBD enable binding to specific DNA sequences(as figure 1 show), so that we can use ZF_21-16-KRAB as a specific transcription factors to repress the expression of our mammalian synthetic promoter SV40-8_ZF_21-16 ( BBa_K2446030).
Figure 1:(A)Design of SynTF-SynPros. (B) The specific binding sites sequences of Zinc finger we used
The information of other SynTF-SynPros is showed in the table below.
| SynTFs | SynPros |
|---|---|
| Gal4-KRAB(TF-KRAB-1) BBa_K2446037 | Sv40-UAS(Sv40-UAS) BBa_K2446036 |
| ZF_PIP_KRAB(TF-KRAB-2) BBa_K2446045 | SV40_2/4/8_PIP BBa_K2446033/BBa_K2446034/BBa_K2446035 |
| ZF_21-16KRAB(TF-KRAB-3) BBa_K2446039 | SV40_8_ZF_21-16 BBa_K2446030 |
| ZF_42-10_KRAB(TF-KRAB-4) BBa_K2446040 | SV40_8_ZF_42-10 BBa_K2446025 |
| ZF_43-8_KRAB(TF-KRAB-5) BBa_K2446041 | SV40_2/4/8_ZF_43-8 BBa_K2446026/BBa_K2446027/BBa_K2446028 |
| ZF_54-8_KRAB(TF-KRAB-6) BBa_K2446042 | SV40_8_ZF_54-8 BBa_K2446029 |
| ZFHD1_KRAB(TF-KRAB-7) BBa_K2446043 | SV40_4_ZFHD1 BBa_K2446032 |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 301
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |