Part:BBa_K2332311
PhoCl, a mammalian photocleavable protein
| PhoCl, a Mammalian Photocleavable Protein | |
|---|---|
| Function | Photocleavable Linker |
| Use in | Mammalian cells |
| Abstraction Hierarchy | Part |
| RFC standard | RFC10, RFC23 & RFC25 compatible |
| Backbone | pSB1C3 |
| Submitted by | [http://2017.igem.org/Team:UCL UCL iGEM 2017] |
As part of the UCL 2017's project "Light-induced Technologies" we investigated light sensitive proteins and their possible applications in synthetic genetic circuits. PhoCl is a novel (April 2017) photocleavable protein engineered from a green-to-red photoconvertible fluorescent protein (FP).
As stated in the original paper: "The photoconversion reaction is a violet light (~400 nm)-induced β-elimination reaction that extends the conjugated system of the chromophore with concomitant cleavage of the polypeptide backbone to form an ~66-residue N-terminal fragment and an ~166-residue C-terminal fragment that remain associated."
The original protein has been engineered by Zhang et al. 2017 (Robert E. Campbell lab). The Campbell lab has sent the original plasmid to the UCL iGEM 2017 team as part of a collaboration and the team has made a BioBrick out of the engineered protein.
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