Coding
TSHantag
Part:BBa_K2404000:Design
Designed by: Ryan Coates Group: iGEM17_Cardiff_Wales (2017-09-12)
A Thyroid Stimulating Hormone antagonist without His-Tags
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 540
Illegal PstI site found at 582 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 540
Illegal PstI site found at 582 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 607
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 540
Illegal PstI site found at 582 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 540
Illegal PstI site found at 582 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 794
Design Notes
We generated this part through the following steps:
- We ordered the TSH gBlock from IDT that included 5' and 3' BsmBI restriction sites
- This was introduced into BBa_P10500 by digestion with BsmBI. This generated a 5' and 3' BsaI sites for level 1 cloning
Source
Synthetic G-block. This modified protein was discovered in a paper by Fares et al in 2001.