Regulatory

Part:BBa_M50048:Design

Designed by: Eleanor Glockner, Jack Andraka   Group: Stanford BIOE44 - S11   (2016-12-10)
Revision as of 08:26, 12 December 2016 by Emrglock (Talk | contribs) (→‎Source)


Atrazine riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We replaced the aptamer in the original riboswitch with an aptamer that would target our molecule of interest, atrazine.

Source

Riboswitch components: https://parts.igem.org/Part:BBa_K784005

Atrazine aptamer: In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine, IJMS International Journal of Molecular Sciences

References

Wang L, Chen W, Xu D, Shim BS, Zhu Y, Sun F, Liu L, Peng C, Jin Z, Xu C, Kotov N. Nano Lett. 2009, 9(12), 4147-4152
Song, Y., Zhang, D., Polizzi, K., Zhang, E., Li, G., & Huang, W. (2012). Bacterial Whole Cell Bioreporters in Environmental Health. Biosensors and Environmental Health, 127-148. doi:10.1201/b12775-9
Park, M., Tsai, S., & Chen, W. (2013). Microbial Biosensors: Engineered Microorganisms as the Sensing Machinery. Sensors, 13(5), 5777-5795. doi:10.3390/s130505777
Rai, N., Ferreiro, A., Neckelmann, A., Soon, A., Yao, A., Siegel, J., . . . Tagkopoulos, I. (2015). RiboTALE: A modular, inducible system for accurate gene expression control. Sci. Rep. Scientific Reports, 5, 10658. doi:10.1038/srep10658