Signalling
Part:BBa_M50027:Design
Designed by: Anthony Agbay, Tyler Dao, Vivek Lam Group: Stanford BIOE44 - S11 (2016-11-28)
LuxS Synthase for Autoinducer-2 Pathway in E. coli
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 50
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 50
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 155
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 50
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 50
Illegal AgeI site found at 253 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
There were no modifications of the original genomic sequence. When using the codon optimization tool from Gene Designer 2.0, there were no alterations required.
Source
The sequence was sourced from Uniprot under the name P45578 (LUXS_ECOLI).