Part:BBa_K1956020

RIBOSOFF with plasmid control

RIBOSOFF 1 with plasmid control

Team IIT Madras 2016's Characterization

Trigger RNA: A part of RNA molecule, which codes for LacI protein.
Switch RNA: It was designed to activate the translation process by allowing the ribosomal machinery to bind to RBS, placed upstream of GFP protein coding part. After interacting with the RNA molecules of lacI gene, RIBOSOFF changes it's confirmation to mask the RBS and represses the translation process.

We performed our RIBOSOFF experiment in E. coli DH5alpha. We transformed our cloned device BBa_K1956020, which has RIBOSOFF_lacI switch under J23106 constitutive promoter, upstream of GFP coding part and RFP generator under lacI+pL promoter.

We prepared two types of cells: 1. BBa_K1956020 in pSB1A2 + BBa_K1956034 in pSB1K3, 2. BBa_K1956020 only.
We grew the cells in LB broth media. After, 16 hrs of growth, we inoculated secondary cultures and collect samples at 12 hour of growth.

Positive Control: Device K1956015, which produces GFP and RFP proteins.
Negative Control: Device K1956034, which produces lacI mRNA molecules.

Below result proves the functioning of RIBOSON device. [[Image:Iitm