Device
Part:BBa_K2120303
Designed by: HongYu Yang Group: iGEM16_BIT-China (2016-10-13)
araC-pBAD+B0034+tetR+B0015
<Description></br> This part is composed of two elements:</br> (1) Arabinose induced promoter pBAD and its inhibitor gene araC BBa_K808000.This part contains the promoter as well as the coding sequence for the repressor araC which is transcribed in the opposite direction. (“upstream”) By binding to L(+)-arabinose, araC changes its conformation. This causes the protein to diffuse from the DNA thereby inducing transcription.</br> (2) Tetracyclines induced promoter pTet's inhibitor gene tetR.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
[edit]
Categories
Parameters
None |