Device

Part:BBa_K2120303

Designed by: HongYu Yang   Group: iGEM16_BIT-China   (2016-10-13)
Revision as of 17:03, 22 October 2016 by Beaz (Talk | contribs)


araC-pBAD+B0034+tetR+B0015

<Description></br> This part is composed of two elements:</br> (1) Arabinose induced promoter pBAD and its inhibitor gene araC BBa_K808000.This part contains the promoter as well as the coding sequence for the repressor araC which is transcribed in the opposite direction. (“upstream”) By binding to L(+)-arabinose, araC changes its conformation. This causes the protein to diffuse from the DNA thereby inducing transcription.</br> (2) Tetracyclines induced promoter pTet's inhibitor gene tetR.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None