Composite

Part:BBa_K1918101:Experience

Designed by: Linghan Wang   Group: iGEM16_Tsinghua-A   (2016-10-14)
Revision as of 07:36, 14 October 2016 by SheepW (Talk | contribs) (→‎Applications of BBa_K1918101)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1918101

1. Use PCR to get the DNA fragment of TRE and EBFP2N(1-154) 2. Ligate the fragment with vector 3. Heat shock transformation 4. Select colonies 5. Purification of plasmid DNA 7. Sequencing 8. Transfer into 293T cell 9. Use Dox to induce the expression of EBFP2N(1-154) 10. FACS

User Reviews

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