Part:BBa_K1918101:Experience

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Applications of BBa_K1918101

Firstly, we need to acquire the elements. We using PCR, Overlap PCR and Gibson to get the elements such as EBFP2, EBFP2N(1-154), EBFP2C(155-238), EBFP2N(1-154):IntN and IntC:EBFP2(155-238).

Secondly, we construct the part. We combine the elements with the vector and transfer the vector into the competent E. coli cells. After that, we select the positive clones and use colony PCR to identify the recombinants. Culturing the positive clones and sequencing the isolated plasmids to make sure that we have get the needed parts.

Lastly, we transfect the plasmids to 289H cells and do the FACS test. In the results of FASC test, the EBFP-A signals tell us that the split florescent proteins could fuse together and our parts work fine.

User Reviews

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