Intermediate

Part:BBa_I759001:Design

Designed by: Josh Michener   Group: iGEM07_Caltech   (2007-06-27)
Revision as of 04:13, 27 October 2007 by Cal (Talk | contribs) (→‎Design Notes)


pTet-spacer-N


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The pTet promoter regulates N transcript production, and transcription can be repressed by tet repressor, or re-activated by the addition of aTc. This construct is an intermediate, as it is missing the terminator for the transcript. The spacer acts as a placeholder for cis sequences which will be cloned in in the future.

Source

Caltech iGEM

References