Part:BBa_I759001:Design

pTet-spacer-N

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The pTet promoter regulates N transcript production, and transcription can be repressed by tet repressor, or re-activated by the addition of aTc. This construct is an intermediate, as it is missing the terminator for the transcript. The spacer acts as a placeholder for cis sequences which will be cloned in in the future.

Source

pTet is a standard iGEM promoter, and the N DNA sequence came from the genome of commercial lambda zap virus. The two parts were connected with an intervening spacer through the process of overlap extension PCRs, using specially designed spacer-primers.