Part:BBa_K1940001

microRNA-34c detector 1.0

We designed this part to detect the abundance of microRNA-34c. Anti-microRNA-34c (BBa_K1940000) was insert after a constitutive promoter (BBa_J23106) which express GFP. As anti- microRNA-34c is sensitive to the expression levels of miR-34c, we would have a direct impact on GFP’s expression. Determining GFP’s fluorescence intensity differences will justify the content amount of microRNA-34c.

Figure 1: Construct design. dCas9 was fused via a 3 amino acid linker to VP16. The resulting fusion construct was flanked by NLS sequences and tagged by a HA epitope. The CMV promoter and BGH terminator were chosen to control gene expression.

Sequence and Features

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Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 186
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 50
  Illegal BsaI.rc site found at 183
  Illegal BsaI.rc site found at 864