Composite
Part:BBa_K1897014
Designed by: Ang Shi Hui Group: iGEM16_NUS_Singapore (2016-10-10)
Revision as of 13:46, 15 October 2016 by Shihuiangle (Talk | contribs)
GFP with pLuxR
This part was made up of promoter pLuxR BBa_R0062, ribosome binding site (RBS) BBa_B0030, green fluorescent protein (GFP) coding sequence BBa_E0040 and double terminator BBa_B0015.
The GFP protein originated from jellyfish Aequeora victoria, and has a dual-peak absorption-excitation spectrum with a major peak at 396 nm and a smaller peak at 475 nm. Excitation at both peaks results in flurorescence at 503 or 509 nm (Mishin et al., 2010).
Usage and Biology
This part can be used to visually verify that the whole quorum sensing system with LuxR (BBa_K1897008) and LuxI (BBa_K1897008) is working, and that the genes under the control of pLuxR are being expressed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 84
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 740
Construction of composite GFP
Verification of composite GFP
[edit]
Categories
Parameters
None |