Part:pSB3C5:Experience
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how you used this part and how it worked out.
Applications of pSB3C5
User Reviews
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pSB3C5 |
This plasmid seems to work. When transformed into TOP10 cells, pSB3C5-I52001 does not transform but when cut with NotI and self-ligated (to eliminate ccdB), lots of colonies are obtained. However, when miniprepped, pSB3C5-I52001 does not give as high of yields as expected for a high copy plasmid likely due to mutations in BBa_I52001 that render it nonfunctional as a high copy origin. I am working to address this issue. This plasmid should still work fine (it is just not as convenient to prep). |
UNIQb38ef10a44af206c-partinfo-00000002-QINU