Part:BBa_K1602046

RRK3

Composite part consisting of an araC-regulated pBAD-promoter (BBa_K808000), Key3 (BBa_J01086) and a terminator (BBa_B0015). It is half of a two-part riboregulator-system for E.coli for posttransciptional regulation of gene expression. Upon transcription the sequence of Key3(taRNA) forms a RNA-RNA-complex with corresponding cis-repressing sequences (crRNA). This leads to a helix shift and the release of the formerly masked ribosome binding site (RBS) enabling the expression of the regulated gene of interest (GOI). The corresponding crRNAs are RRL3H and RRL3G.

Figure 1: Interaction of taRNA and crRNA leads to gene expression.

Because of the araC-regulated pBAD-promoter production of the Lock3-sequence is induced by the addition of arabniose. In the presence of glucose araCpBAD shows a very low basal expression level. In combination with the corresponding crRNA-parts (RRL3H and RRL3G) this results in an induceable riboregulator-system which represses gene expression in the presence of glucose but is inducable through the addition of arabinose.

References

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1215
  Illegal SapI site found at 961