Translational_Unit

Part:BBa_K1602014

Designed by: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun   Group: iGEM15_TU_Darmstadt   (2015-09-16)
Revision as of 22:00, 18 September 2015 by Henklem (Talk | contribs)

B0034-yagE

This composite represents the 2-dehydro-3-deoxy-D-pentonate aldolases yagE plus the ribosomal binding site B0034.

Usage

This part is a composite of one coding gene and a strong RBS (BBa_B0034).

his part makes it possible to establish an ethyleneglycol producing pathway in other hosts than E. coli if its combined with other enzymes of the pathway (BBa_K1602017) (BBa_K1602002) (BBa_K1602015)

  • yagE
  • RBS-B0034
https://static.igem.org/mediawiki/parts/8/8e/TU_Darmstadt_plasmid-card_B0034-yagE.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 193
[edit]
Categories
Parameters
None