DNA

Part:BBa_K1732007

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-09)
Revision as of 01:08, 17 September 2015 by Donnalee (Talk | contribs)

pSB1C3-J23100-B0034-BFP-His-B0015

pSB1C3-J23100-B0034-BFP-His-B0015

His tagged mTagBFP and strong RBS. The J23100 strong constitutive promoter allows for high level expression of the fluorescent protein used to measure fluorescence.

A set of fluorescent proteins which includes BFP, GFP, eGFP, E0040, OFP, YFP, and RFP with a J23100 constitutive promoter and RFP with a J23115 constitutive promoter were characterized. The relative fluorescence as well as the signal-to-noise ratio of all the proteins in the MACH competent cell line were calculated. BFP was measured at (ex/em = 399nm/456).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 285
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 607
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 518
    Illegal BsaI.rc site found at 683


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Categories
Parameters
None