Coding
Part:BBa_K1629000
Designed by: Juan Carlos Rueda Silva, Daniela Flores Gomez, Oliva Snchez Group: iGEM15_TecCEM_HS (2015-08-26)
Light chain-heavy chain ferritin dimer with Histag 6X
Description to be uploaded.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1098
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 575
Illegal NgoMIV site found at 1040
Illegal NgoMIV site found at 1159
Illegal NgoMIV site found at 1223
Illegal NgoMIV site found at 1492 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 416
Illegal SapI.rc site found at 427
Illegal SapI.rc site found at 1540
Results
iGEM Groningen 2015 did an experiment with this construct to confirm it's expression. In the picture below is the expression of the protein visible. When comparing the bands in the control and the IPTG induction groups, a clear expression of a protein the size of ferritin can be observed.
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Categories
Parameters
None |