Coding

Part:BBa_K1629000

Designed by: Juan Carlos Rueda Silva, Daniela Flores Gomez, Oliva Snchez   Group: iGEM15_TecCEM_HS   (2015-08-26)
Revision as of 20:00, 15 September 2015 by HarmRuesink (Talk | contribs) (→‎Results)

Light chain-heavy chain ferritin dimer with Histag 6X

Description to be uploaded.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1098
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 575
    Illegal NgoMIV site found at 1040
    Illegal NgoMIV site found at 1159
    Illegal NgoMIV site found at 1223
    Illegal NgoMIV site found at 1492
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 416
    Illegal SapI.rc site found at 427
    Illegal SapI.rc site found at 1540


Results

iGEM Groningen 2015 did an experiment with this construct to confirm it's expression. In the picture below is the expression of the protein visible. When comparing the bands in the control and the IPTG induction groups, a clear expression of a protein the size of ferritin can be observed.

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Categories
Parameters
None