Coding

Part:BBa_K1629000

Designed by: Juan Carlos Rueda Silva, Daniela Flores Gomez, Oliva S�nchez   Group: iGEM15_TecCEM_HS   (2015-08-26)

Light chain-heavy chain ferritin dimer with Histag 6X

Ferritin is an enzyme that captures iron ions in blood. It has been found that SDS molecules can bind between ferritin subunits. Although it's not the main function of this enzyme, team [http://2015.igem.org/Team:TecCEM_HS TecCEM_HS 2015] uses this enzyme to capture SDS molecules solution. This biobrick was based on a similar design from iGEM [http://2013.igem.org/Team:Calgary Team Calgary 2013].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1098
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 575
    Illegal NgoMIV site found at 1040
    Illegal NgoMIV site found at 1159
    Illegal NgoMIV site found at 1223
    Illegal NgoMIV site found at 1492
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 416
    Illegal SapI.rc site found at 427
    Illegal SapI.rc site found at 1540


Results

iGEM Groningen 2015 did an experiment with this construct to confirm it's expression. In the picture below is the expression of the protein visible. When comparing the bands in the control and the IPTG induction groups, a clear expression of a protein the size of ferritin can be observed.

References

Liu, R. et. al. (May 2012) Beyond the detergent effect: a binding site for sodium dodecyl sulfate (SDS) in mammalian apoferritin. Retrieved from: http://www.ncbi.nlm.nih.gov/pubmed/22525747

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