Composite

Part:BBa_M36019:Experience

Designed by: Rachel Lim   Group: Stanford BIOE44 - S11   (2014-10-23)
Revision as of 23:36, 5 December 2014 by Rachelim (Talk | contribs) (→‎Applications of BBa_M36019)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_M36019

Stanford Location By: Melissa, Sydney, Rachel (BIOE 44 Fall 2014) Experience:

From fluorescence assay conducted, we found that the sensor failed to perform its intended function (detecting lactic acid). Fluorescence/OD measurement remained constant across lactic acid concentrations on a log scale from 0 to 0.1M. We suspect that it is because E. coli themselves regulate lactate in the environment, hence over time the lactate concentration equilibrates to a constant concentration across all experimental set ups. Assay results.png

- Barcode # 0133010776, 0133010749, 0133024645, 0133009701, 0133009727

- Plasmid name: somcrl1

- Antibiotic resistance: amp

- DNA 2.0 gene # 193463

- Organism expressed in: E. Coli

- Sensor for lactic acid

User Reviews

UNIQ7bbd7c26db82bb83-partinfo-00000000-QINU UNIQ7bbd7c26db82bb83-partinfo-00000001-QINU