Composite

Part:BBa_M36019:Experience

Designed by: Rachel Lim   Group: Stanford BIOE44 - S11   (2014-10-23)

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Applications of BBa_M36019

Stanford Location By: Melissa, Sydney, Rachel (BIOE 44 Fall 2014) Experience:

From fluorescence assay conducted, we found that the sensor failed to perform its intended function (detecting lactic acid). Fluorescence/OD measurement remained constant across lactic acid concentrations on a log scale from 0 to 0.1M. We suspect that it is because E. coli themselves regulate lactate in the environment, hence over time the lactate concentration equilibrates to a constant concentration across all experimental set ups.

Assay results.png

- Barcode # 0133010776, 0133010749, 0133024645, 0133009701, 0133009727

- Plasmid name: somcrl1

- Antibiotic resistance: amp

- DNA 2.0 gene # 193463

- Organism expressed in: E. Coli

- Sensor for lactic acid

User Reviews

UNIQ50da7c16bd696664-partinfo-00000000-QINU UNIQ50da7c16bd696664-partinfo-00000001-QINU