Generator
SG

Part:BBa_J45320

Designed by: André Green II   Group: iGEM06_MIT   (2006-10-20)
Revision as of 23:02, 25 January 2008 by Rshetty (Talk | contribs)

Salicylate generator

Protein generator converting PoPS to the individual pchA and pchB proteins. These two proteins biosynthesize salicylic acid. Although the coding regions overlap, there is no fusion protein; the ribosome binding site for pchA is located at the end of the coding region of pchB. This part is almost identical to BBa_J45300, except that it uses the ribosome binding site BBa_B0032 instead of BBa_B0030 (which is in BBa_J45300).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 389
    Illegal NgoMIV site found at 793
    Illegal NgoMIV site found at 918
    Illegal NgoMIV site found at 929
    Illegal NgoMIV site found at 1210
    Illegal NgoMIV site found at 1678
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/generator/prct
//function/odor
s
Parameters
o_h
proteinPchA & PchB
rbs
tagNone