Regulatory

Part:BBa_K1372001

Designed by: Sylvie Lautru Fabio Maschi   Group: iGEM14_Paris_Saclay   (2014-10-02)
Revision as of 19:20, 17 October 2014 by Maher (Talk | contribs)

Salicylate Inducible Suppressing System

This BB is the result of the assembly of two BB:

- BBa_K1372000 (lien):

Paris Saclay BB2000.png

- BBa_B0015 (lien): Double terminator consisting of BBa_B0010 and BBa_B0012.

Thus, there will be a terminator of the transcription of tRNA-SUPD gene (missing in BBa_K1372000).

Paris Saclay BB2001.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 77
    Illegal NgoMIV site found at 618
    Illegal AgeI site found at 1309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1312


[edit]
Categories
Parameters
None