Coding

Part:BBa_K1498000:Experience

Designed by: Charlotte Green   Group: iGEM14_Sheffield   (2014-10-07)
Revision as of 18:41, 17 October 2014 by Charlieinabox (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1498000

Under the control of the constitutive promoter BBa_J23110 and following expression in E. coli BL21 the supernatant was analysed on a SDS-PAGE gel. Following coomassie staining a protein of 36.5 kDa was visible, suggesting that the pre-processed protein was present. However only the processed form of the protein will exhibit activity.

In theory BBa_K1498000 should be suitable to use in assays to degrade keratin rich structures (hair, nails, feathers).

As a protease gelatin degredation should also be observed.

User Reviews

UNIQe57c2284a0707aca-partinfo-00000000-QINU UNIQe57c2284a0707aca-partinfo-00000001-QINU