Coding

Part:BBa_K1497024

Designed by: Sascha Hein, Rene Sahm   Group: iGEM14_TU_Darmstadt   (2014-10-07)
Revision as of 12:14, 11 October 2014 by N.Hummel (Talk | contribs)

GBD-Domain

The GBD domain and its ligand (BBa_K771106) are suitable tools for protein colocalizaion. Initially, the domain was a part of the N-WASP protein (GTPase binding domain) in Rattus rattus. The GBD domain is used as a binding unit of the so-called protein scaffold published by Dueber et al. in 2012. The scaffold (BBa_K1497033) is composed of different binding units, which enable the assembly of multiple target proteins. This BioBrick adds a BglII and BamHI restriction site in front of and behind the previously constructed domain sequence (BBa_K771105). Now, different binding units of the scaffold protein can be fused together without the introduction of restriction sites. This allows the easy construction of BioBricks of different permutations of the scaffold protein domains.


Figure 1 .
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None