Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Device

Part:BBa_K1355003

Designed by: Maria Clara Tavares Astolfi, Luna Barroco de Lacerda   Group: iGEM14_UFAM_Brazil   (2014-10-06)
Revision as of 21:45, 11 October 2014 by Mctastolfi (Talk | contribs)

Mercury ions accumulator device


The Bioaccumulative Biobrick is composed of our “Essential biobrick” (BBa_K1355001) + a Metallothionein (BBa_K346004) available in the database of the iGEM since 2010 ,as represented below:

L5.jpg

In mercury’s presence, MerR protein will bind to it so MerT, MerP and Metal Binding Protein (MBP) proteins are synthesized and bioaccumulation will start! MerP and MerT protein are responsible for mercury’s transport of mercury from the periplasm to cytoplasm, leading it to MBP! The MBP binds to the mercury in the cytoplasm, inactivating it.

File:MercuryBacterBIoacc.jpg

Figure 1: Mercury Bacter Hg bioaccumulator (DH5-alpha transformed with BBa_K1355003)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 988
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 586
    Illegal NgoMIV site found at 1160
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 579


[edit]
Categories
Parameters
None